Getting My cytometers To Work
Getting My cytometers To Work
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Data-enriched characterization of mobile populations. Morphological information from illustrations or photos can increase to your richness of flow cytometry details. By way of example, the figure displays an normally standard apoptosis assay applying Annexin V and PI, including mobile imaging to characterize cells in Every populace to expose morphologically distinct attributes. These insights couldn't are actually received from multiplex staining by itself.
Whether you’re a rookie or a professional, explore our methods to progress your investigation and expertise in flow cytometry.
Merge acoustic concentrating technology with superior-parameter analysis for adaptable spectral unmixing and conventional workflows.
Find out new insights into your experimental details by visualizing populations employing combined fluorescence and morphology data. Our software package interface wizards help downsampling, concatenation (merged files) and dimensionality resources which are crafted in to the computer software for an intuitive, streamlined info Examination expertise.
"An image is not quite worthy of a thousand dots, but it surely definitely helps you to see photos. It is excellent to find out pictures that correlate with staining. For example, AnnexinV-beneficial cells that happen to be morphologically various than healthful cells, or CD14+ monocytes which have been more substantial and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a highly skilled consumer has gated singlets confidently. Right after analyzing the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate incorporates much more than four% aggregates.
Optimizing gating tactic. Even robust manual singlet gating is error-vulnerable and continues to be a subjective conclusion stage in Nearly all flow cytometry assays. Imaging can be used to verify and change gates to incorporate only solitary cells of fascination.
Dependable graphic high quality even at high throughput Acoustic focusing and a superior-pace digital camera Incorporate to image these T cells constantly at minimal or substantial flow fees. Effortlessly alter target and digicam options to satisfy experimental needs.
The Attune NxT cytometer is the reputable workhorse instrument your lab can trust in for consistent and trustworthy effectiveness. The Attune CytPix design features a higher-pace brightfield camera and AI-pushed image Assessment software, with recent upgrades together with device Mastering resources and an intuitive consumer interface, enabling Improved data precision, precision, and quicker insights into sample biology.
Perhaps most significantly, these events comprise cells of Evidently various phenotypes which could lead on to incorrect conclusions concerning double good gatherings (specifically in uncommon populations).
Sound waves enable specific mobile alignment, causing Fantastic knowledge high-quality and ~10X a lot quicker sample flow costs.
Application note: Automated impression Investigation lessens consumer-to-user variability in flow cytometry gating methods
Leverage the strength of Superior technological innovation and innovation to accelerate your discoveries. Trust in the Attune Flow Cytometer relatives to deliver the reliability, speed, and precision that established your perform apart.
Adaptable payment strategy from Agilent Fiscal Methods allow you to obtain the essential instrumentation get more info you will need for fast task, or to develop your lab.
At the heart of every Attune Flow Cytometer lies our revolutionary acoustic focusing technology, which makes use of seem waves to meticulously align cells inside the flow mobile. This ground breaking technique surpasses classic hydrodynamic techniques, leading to quicker processing speeds, Remarkable info high quality, and enhanced sample throughput.
The Attune Xenith cytometer is often a superior-parameter spectral analyzer, offering each spectral unmixing and traditional flow abilities for detailed and adaptable mobile Assessment.
Agilent Price Guarantee guarantees you a minimum of 10 years of instrument use from the date you buy. In any other case, we will credit rating you Along with the residual price of the technique towards an upgraded model.
What seemed to be a homogeneous population is definitely a lot more varied—an Perception that should be viewed as when interpreting results.